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EIF4A2 Rabbit pAb

Anti-EIF4A2 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S218874

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S218874
产品全名:   EIF4A2 兔多抗
基因符号   DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
UNIPROT ID:   Q14240 (Gene Accession – BC015842 )
背景:   ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5′-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
抗原:   Fusion protein of human EIF4A2
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 218874(EIF4A2 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the fusion protein and then with 218874(Anti-EIF4A2 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse brain tissue, K562 and Raji cell lysates;
Primary antibody: 218874(EIF4A2 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
           引用文献