| 货号: S219757 |
| 产品全名: EGR4 兔多抗 |
| 基因符号 NGFIC, NGFI-C, PAT133 |
| UNIPROT ID: Q05215 (Gene Accession – NP_001956 ) |
| 背景: Early growth response protein 4 (EGR-4), also known as AT133, is a protein that in humans is encoded by the EGR4 gene. EGR-4 is a member of the early growth response (EGF) family of zinc finger transcription factors. Transcriptional regulator. Recognizes and binds to the DNA sequence 5′-GCGGGGGCG-3′ (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. |
| 抗原: Synthetic peptide of human EGR4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219757(EGR4 Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 219757(Anti-EGR4 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 219757(Anti-EGR4 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260293(Anti-EGR4 Antibody) at dilution 1/20. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human fetal brain tissue, Mouse brain tissue;
Primary antibody: 219757(EGR4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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