| 货号: S210287 |
| 产品全名: E2F6 兔多抗 |
| 基因符号 E2F-6 |
| UNIPROT ID: O75461 (Gene Accession – BC008348 ) |
| 背景: This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. |
| 抗原: Fusion protein of human E2F6 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 210287(E2F6 Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 210287(Anti-E2F6 Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 210287(Anti-E2F6 Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D120574(Anti-E2F6 Antibody) at dilution 1/60. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: LoVo cells, A549 cells, human hepatocellular carcinoma tissue, Jurkat cells, Hela cells;
Primary antibody: 210287(E2F6 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds | |
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