| 货号: S216501 |
| 产品全名: DUSP4 兔多抗 |
| 基因符号 TYP, HVH2, MKP2, MKP-2 |
| UNIPROT ID: Q13115 (Gene Accession – BC002671 ) |
| 背景: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. |
| 抗原: Fusion protein of human DUSP4 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-150;WB: 500-2000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 216501(DUSP4 Antibody) at a dilution of 1/30(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 216501(Anti-DUSP4 Antibody) at dilution 1/30. | 
Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane: RAW264.7 cells;
Primary antibody: 216501(DUSP4 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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