| 货号: S216476 |
| 产品全名: DNMT3A 兔多抗 |
| 基因符号 TBRS; DNMT3A2; M.HsaIIIA |
| UNIPROT ID: Q9Y6K1 (Gene Accession – BC043617 ) |
| 背景: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. |
| 抗原: Fusion protein of human DNMT3A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216476(DNMT3A Antibody) at a dilution of 1/80(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216476(Anti-DNMT3A Antibody) at dilution 1/80. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 216476(Anti-DNMT3A Antibody) at a dilution of 1/80. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D220559(Anti-DNMT3A Antibody) at dilution 1/80. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and 231 cell lysates;
Primary antibody: 216476(DNMT3A Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes | |
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