| 货号: S217983 |
| 产品全名: DLAT 兔多抗 |
| 基因符号 DLTA; PDCE2; PDC-E2 |
| UNIPROT ID: P10515 (Gene Accession – BC039084 ) |
| 背景: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood. |
| 抗原: Fusion protein of human DLAT |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 30-150;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217983(DLAT Antibody) at a dilution of 1/40(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217983(Anti-DLAT Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 217983(Anti-DLAT Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with fusion protein and then with D223495(Anti-DLAT Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A431, hepg2 and lncap cell;
Primary antibody: 217983(DLAT Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds | |
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