| 货号: S217355 |
| 产品全名: DDX43 兔多抗 |
| 基因符号 CT13; HAGE |
| UNIPROT ID: Q9NXZ2 (Gene Accession – BC066938 ) |
| 背景: DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX43 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 43), also known as CT13 or HAGE, is a 648 amino acid protein that contains one KH domain, one helicase C-terminal domain and one helicase ATP-binding domain and belongs to the DEAD-box family. Expressed in testis and present at abnormally high levels in a variety of tumors, DDX43 is thought to function as an ATP-dependent RNA helicase that may play a role tumor transformation and metastasis. |
| 抗原: Fusion protein of human DDX43 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217355(DDX43 Antibody) at a dilution of 1/40(Cytoplasm or Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217355(Anti-DDX43 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217355(Anti-DDX43 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222223(Anti-DDX43 Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cells;
Primary antibody: 217355(DDX43 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes | |
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