| 货号: N262123 |
| 产品全名: DDX21 兔单克隆抗体 |
| 基因符号 GUA; GURDB; RH-II/GU; RH-II/GuA |
| UNIPROT ID: Q9NR30 |
| 背景: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) (PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs (PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2′-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes (PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes (PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at ‘Ser-77’ (PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase) (PubMed:9461305). Involved in rRNA processing (PubMed:14559904, PubMed:18180292). May bind to specific miRNA hairpins (PubMed:28431233). |
| 抗原: A synthetic peptide of human DDX21 |
| 经过测试的应用: WB,ICC/IF |
| 推荐稀释比: WB: 1/500-1/1000 IF: 1/50-1/200 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R04-4J2 |
| 分子量: Calculated MW: 87 kDa; Observed MW: 87 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of DDX21 in Hela lysates using DDX21 antibody. | 
Immunocytochemistry analysis of DDX21 (green) in 293T using DDX21 antibody,and DAPI(blue). |
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