| 货号: S219738 |
| 产品全名: DDB1 兔多抗 |
| 基因符号 XPE, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1 |
| UNIPROT ID: Q16531 (Gene Accession – NP_001914 ) |
| 背景: The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) – an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. |
| 抗原: Synthetic peptide of human DDB1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 10-100;WB: 200-1000;ELISA: 500-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 219738(DDB1 Antibody) at a dilution of 1/5(Cytoplasm, Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 219738(Anti-DDB1 Antibody) at dilution 1/5. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: MCF7 cells, Hela cells, HepG2 cells;
Primary antibody: 219738(DDB1 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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