| 货号: S211080 |
| 产品全名: CYFIP2 兔多抗 |
| 基因符号 PIR121 |
| UNIPROT ID: Q96F07 (Gene Accession – BC011762 ) |
| 背景: CYFIP2 (Cytoplasmic FMR1-interacting protein 2, p53-inducible protein 121) is a 1,278 amino acid protein encoded by the human gene CYFIP2. CYFIP2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. It interacts with FMR1, FXR1 and FXR2 and is a component of the WAVE1 complex composed of Abi-2, CYFIP2, C3orf10/HSPC300, NAP125 and WASF1/WAVE1. Upon binding to activated Rac 1, CYFIP2 causes the complex to dissociate, releasing activated WASF1. The CYFIP2 promoter contains a p53-responsive element that confers p53 binding as well as transcriptional activation of a heterologous reporter. Induced expression of CYFIP2 is sufficient for caspase activation and cellular apoptosis, reminiscent of p53 activation. |
| 抗原: Fusion protein of human CYFIP2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer, Neuroscience, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 211080(CYFIP2 Antibody) at a dilution of 1/25(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 211080(Anti-CYFIP2 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 211080(Anti-CYFIP2 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D122175(Anti-CYFIP2 Antibody) at dilution 1/25. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Mouse brain tissue, human fetal brain tissue;
Primary antibody: 211080(CYFIP2 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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