| 货号: S221821 |
| 产品全名: COX6A2 兔多抗 |
| 基因符号 COX6AH; COXVIAH |
| UNIPROT ID: Q02221 (Gene Accession – NP_005196 ) |
| 背景: Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity. |
| 抗原: Synthetic peptide of human COX6A2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 221821(COX6A2 Antibody) at a dilution of 1/20(Mitochondrion inner membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 221821(Anti-COX6A2 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 221821(Anti-COX6A2 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D263581(Anti-COX6A2 Antibody) at dilution 1/20. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human muscle tissue, Human heart tissue, Mouse heart tissue lysates;
Primary antibody: 221821(COX6A2 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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