| 货号: S218220 |
| 产品全名: CISH 兔多抗 |
| 基因符号 CIS; G18; SOCS; CIS-1; BACTS2 |
| UNIPROT ID: Q9NSE2 (Gene Accession – BC031590 ) |
| 背景: The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Fusion protein of human CISH |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218220(CISH Antibody) at a dilution of 1/30(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218220(Anti-CISH Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218220(Anti-CISH Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D223972(Anti-CISH Antibody) at dilution 1/30. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human kidney tissue lysate;
Primary antibody: 218220(CISH Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes | |
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