| 货号: S216416 |
| 产品全名: CEACAM8 兔多抗 |
| 基因符号 CD67; CGM6; CD66b; NCA-95 |
| UNIPROT ID: P31997 (Gene Accession – BC026263 ) |
| 背景: Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764). By probing white blood cell cDNA libraries with tumor nonspecific crossreacting antigen, Arakawa et al. (1990) isolated a cDNA encoding CEACAM8, which they called NCA-W272. The deduced 349-amino acid protein contains 11 potential N-glycosylation sites and 4 cysteines expected to form 2 intramolecular disulfide linkages. It also has a signal peptide and several domains similar to those of other CEA family proteins, such as CEACAM1 (109770). Immunoblot analysis showed expression of a 70-kD protein that was reduced to a 37-kD major protein and a 34-kD minor protein in the presence of a glycosylation inhibitor. |
| 抗原: Fusion protein of human CEACAM8 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 216416(CEACAM8 Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 216416(Anti-CEACAM8 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 216416(Anti-CEACAM8 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D220405(Anti-CEACAM8 Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A431 and HepG2 cell lysates;
Primary antibody: 216416(CEACAM8 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes | |
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