| 货号: S220449 |
| 产品全名: CDC34 兔多抗 |
| 基因符号 UBC3; UBCH3; UBE2R1; E2-CDC34 |
| UNIPROT ID: P49427 (Gene Accession – NP_004350 ) |
| 背景: The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein comple,x which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. |
| 抗原: Synthetic peptide of human CDC34 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-300;WB: 500-2000;ELISA: 2000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human Lymphoma tissue using 220449(CDC34 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human Lymphoma tissue is first treated with the synthetic peptide and then with 220449(Anti-CDC34 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220449(Anti-CDC34 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261544(Anti-CDC34 Antibody) at dilution 1/50. | 
Gel: 15%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, A431 cells;
Primary antibody: 220449(CDC34 Antibody) at dilution 1/900;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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