| 货号: S218453 |
| 产品全名: CDC23 兔多抗 |
| 基因符号 APC8; CUT23; ANAPC8 |
| UNIPROT ID: Q9UJX2 (Gene Accession – BC017713 ) |
| 背景: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. |
| 抗原: Fusion protein of human CDC23 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 20-100;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cancer, Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218453(CDC23 Antibody) at a dilution of 1/20(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218453(Anti-CDC23 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218453(Anti-CDC23 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224484(Anti-CDC23 Antibody) at dilution 1/20. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, SKOV3 and Hela cell lysates;
Primary antibody: 218453(CDC23 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds | |
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