| 货号: S220112 |
| 产品全名: CD63 兔多抗 |
| 基因符号 MLA1; ME491; LAMP-3; OMA81H; TSPAN30 |
| UNIPROT ID: P08962 (Gene Accession – NP_001244318 ) |
| 背景: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. |
| 抗原: Synthetic peptide of human CD63 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cardiovascular, Immunology, Cell Markers, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 220112(CD63 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 220112(Anti-CD63 Antibody) at dilution 1/50. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: LNCAP cell lysate;
Primary antibody: 220112(CD63 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds | |
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