| 货号: S217201 |
| 产品全名: BCAS2 兔多抗 |
| 基因符号 DAM1; SPF27; Snt309 |
| UNIPROT ID: O75934 (Gene Accession – BC005285 ) |
| 背景: BCAS2 (breast carcinoma amplified sequence 2), also designated DAM1 (DNA amplified in mammary carcinoma 1 protein) or spliceosome-associated SPF 27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. BCAS2 is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TR? (thyroid hormone receptor beta), ER (estrogen receptor alpha), ER?, PR (progesterone receptor), and PPAR? (Peroxisome proliferator-activated receptor gamma). BCAS2 functions as an ER coactivator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer. |
| 抗原: Fusion protein of human BCAS2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-200;WB: 200-1000;ELISA: 1000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217201(BCAS2 Antibody) at a dilution of 1/50(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217201(Anti-BCAS2 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 217201(Anti-BCAS2 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D221974(Anti-BCAS2 Antibody) at dilution 1/50. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A172 cells, 231 cells;
Primary antibody: 217201(BCAS2 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
|
