| 货号: S219098 |
| 产品全名: ATP6V1C1 兔多抗 |
| 基因符号 VATC; Vma5; ATP6C; ATP6D |
| UNIPROT ID: P21283 (Gene Accession – BC010960 ) |
| 背景: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c’, c’, and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D. |
| 抗原: Fusion protein of human ATP6V1C1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 100-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219098(ATP6V1C1 Antibody) at a dilution of 1/100(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 219098(Anti-ATP6V1C1 Antibody) at dilution 1/100. | 
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 219098(Anti-ATP6V1C1 Antibody) at a dilution of 1/100. | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D225827(Anti-ATP6V1C1 Antibody) at dilution 1/100. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human cerebella tissue and Human cerebrum tissue lysates;
Primary antibody: 219098(ATP6V1C1 Antibody) at dilution 1/500;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds | |
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