| 货号: S220314 |
| 产品全名: ATG4A 兔多抗 |
| 基因符号 APG4A; AUTL2 |
| UNIPROT ID: Q8WYN0 (Gene Accession – NP_443168 ) |
| 背景: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. |
| 抗原: Synthetic peptide of human ATG4A |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 200-1000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer, Cell Biology, Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220314(ATG4A Antibody) at a dilution of 1/30(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220314(Anti-ATG4A Antibody) at dilution 1/30. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220314(Anti-ATG4A Antibody) at a dilution of 1/30. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D261325(Anti-ATG4A Antibody) at dilution 1/30. | 
Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 231 cells, human liver cancer tissue;
Primary antibody: 220314(ATG4A Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes | |
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