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ANAPC2 Rabbit pAb

Anti-ANAPC2 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S217013

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S217013
产品全名:   ANAPC2 兔多抗
基因符号   APC2; RP11-350O14.5
UNIPROT ID:   Q9UJX6 (Gene Accession – BC032503 )
背景:   A large protein comple,x termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.
抗原:   Fusion protein of human ANAPC2
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cancer, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 217013(ANAPC2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 217013(Anti-ANAPC2 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Hela cells, Jurkat cells, NIH/3T3 cells;
Primary antibody: 217013(ANAPC2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
           引用文献