| 货号: N261810 |
| 产品全名: AKT 兔单克隆抗体 |
| 基因符号 MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma |
| UNIPROT ID: Q9Y243 |
| 背景: AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis. |
| 抗原: A synthetic peptide of human AKT1/2/3 |
| 经过测试的应用: WB,ICC/IF |
| 推荐稀释比: WB: 1/500-1/1000 IF: 1/50-1/200 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R08-8F1 |
| 分子量: Calculated MW: 56 kDa; Observed MW: 56 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human, Mouse and Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: apoptosis – Bcl-2 family |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunocytochemistry analysis of AKT1/2/3(green) in Hela using AKT antibody,and DAPI(blue) | 
Western blot analysis of AKT in 3T3, rat heart lysates using AKT antibody. | 
Western blot analysis of AKT1/2/3 in LnCap lysates using AKT antibody. | |
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