| 货号: S216987 |
| 产品全名: ACTN1 兔多抗 |
| 基因符号 BDPLT15 |
| UNIPROT ID: P12814 (Gene Accession – BC003576 ) |
| 背景: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Fusion protein of human ACTN1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 15-50;WB: 500-2000;ELISA: 2000-5000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216987(ACTN1 Antibody) at a dilution of 1/10(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216987(Anti-ACTN1 Antibody) at dilution 1/10. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216987(Anti-ACTN1 Antibody) at a dilution of 1/10. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D221591(Anti-ACTN1 Antibody) at dilution 1/10. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 231, A549 and Hela cell, Human fetal muscle tissue lysates;
Primary antibody: 216987(ACTN1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds | |
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