蛋白质印迹:   

Western blot analysis of recombinant ALK protein. Purified His-tagged ALK protein was blotted with Anti-ALK 兔多抗 (货号:21280).
Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Anti-ALK 兔多抗 (货号:21280).

Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-Rabbit IgG (1:50 dilution) was used as secondary antibody.