武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: AKT1(E49K) 小鼠单抗 |
货号: 26137 |
规格: 100 µl |
基因符号: AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
描述: AKT1(E49K) 小鼠单抗 |
背景: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers. |
免疫原: A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin. |
经过测试的应用: ELISA, WB, IHC |
推荐稀释比: ELISA: 1:1000-1:5000WB: 1:500-1:2000IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯化: Purified from ascites |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes E49K mutant, but not wild type AKT1 of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹: Western blot analysis of recombinant AKT1(E49K) and wild type proteins.Purified His-tagged AKT1(E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-AKT1(E49K) monoclonal antibody (货号:26137). |
Immunofluorescence: Immunofluorescence of cells expressing AKT1 proteins with Anti-AKT1(E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP-AKT1 (WT) plasmid (left column) or pCDNA3-GFP-AKT1(E49K) plasmid (right column), then fixed and stained with Anti-AKT1(E49K) monoclonal antibody (货号:26137). |