| 货号: 28377 |
| 产品全名: 4-1BB Ligand(DM68) 兔单克隆抗体 |
| 基因符号 4-1BB Ligand;TNFSF9;CD137L |
| 描述: 4-1BB Ligand antibody(DM68) 兔单克隆抗体 |
| 背景: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9:4-1BB; which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9:4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene; TNFSF9:4-1BBL; has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines; and is thought to be involved in T cell-tumor cell interaction. |
| 经过测试的应用: ELISA; Flow Cyt |
| 推荐稀释比: ELISA 1:5000-10000; Flow Cyt 1:100 |
| 种属反应性: Rabbit |
| 亚型: Rabbit IgG |
| 纯化: Purified from cell culture supernatant by affinity chromatography |
| 种属反应性: 人 4-1BB Ligand |
| 成分: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human 4-1BB Ligand protein, mFc-His tagged protein 11178 can bind Rabbit anti-4-1BB Ligand monoclonal antibody ( clone: DM68) in a linear range of 1-100 ng/ml. | 
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 4-1BB Ligand (B) were surface stained with Rabbit anti-4-1BB Ligand monoclonal antibody 1µg/ml ( clone: DM68) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. | 
Figure 3. Flow cytometry data of serially titrated Rabbit anti-4-1BB Ligand monoclonal antibody ( clone: DM68) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | |
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