武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: Gα13-GTP 小鼠单抗 |
货号: 货号:: 26902 |
规格: 30 µL |
基因符号: Gna13 |
描述: Gα13-GTP 小鼠单抗 |
背景: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
免疫原: Recombinant full length active Gα13 蛋白 |
经过测试的应用: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG2b |
纯化: Purified from ascites |
Preservative: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹: IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP 小鼠单抗 (货号: 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 小鼠单抗 (货号: 26004). |
Immunohistochemistry: Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells. |
1. Elabela-APJ axis attenuates cerebral ischemia/reperfusion injury by inhibiting neuronal ferroptosis Free Radic Biol Med. 2023 PMID: 36681202 |
2. The GABA B receptor mediates neuroprotection by coupling to G 13 Sci Signal. 2021 PMID: 34665640 |
3. Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor Thromb Res. 2017 PMID: 28609704 |
4. Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization J Biol Chem. 2011 PMID: 21771786 |
5. Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2 Br J Pharmacol. 2011 PMID: 20958291 |
6. Ga12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation Carcinogenesis. 2010 PMID: 20478922 |
7. Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency J Biol Chem. 2009 PMID: 19654325 |