武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: Gαi-GTP 小鼠单抗 |
货号: 26901 |
基因符号: Gnai |
描述: Gαi-GTP 小鼠单抗 |
背景: Heterotrimeric G proteins are critical cellular signal transducers. Gαirepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored. |
免疫原: Recombinant full length protein of active Gα i1 |
经过测试的应用: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG1 |
纯化: Purified from ascites |
Preservative: No |
成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹: Gαi activation assay.A. CHO cells were transfected with wild type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (货号:26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (货号: 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (货号: 26003) of the cell lysates.B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (货号: 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i 兔多抗 (货号: 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates. |