武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处
武汉费斯德生物科技有限公司是美国NewEast Biosciences在中国的办事处。NewEast Biosciences 在十二年前率先研发俩种独特的抗体。这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene。 GTP酶涉及(1)响应细胞表面受体激活的信号转导,包括跨膜受体,例如介导味觉、嗅觉和视觉的那些,(2)核糖体的蛋白质生物合成,(3)调节细胞分化、增殖、分裂和运动,(4)蛋白质通过膜的易位,(5)细胞内囊泡的运输,以及囊泡介导的分泌和摄取,通过GTP酶控制囊泡外壳组装。Oncogene侧是诱发癌症的基因。
我公司将向你提供以下的独一无二的三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力(无需乙酰化)ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。
¥4,800.00
产品名称: Gαo-GTP 小鼠单抗 |
货号: 26907 |
规格: 30 µL |
基因符号: Gnao |
描述: Anti Gαo-GTP 小鼠单抗 |
背景: Heterotrimeric G proteins are critical cellular signal transducers. Gαorepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored. |
免疫原: Recombinant full length protein of active Gαo |
经过测试的应用: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
种属反应性: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG1 |
纯化: Purified from ascites |
Preservative: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: active Gαo antibody recognizes active Gαo of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹: Gαo Activation Assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (货号: 26907). Immunoblot was with an anti-Gαo兔多抗 (货号: 21015). |
1. The Inhibitory Role of Hydrogen Sulfide in UII-Induced Cardiovascular Effects and the Underlying Signaling Pathways Antioxidants (Basel). 2022 PMID: 36421438 |
2. Golgi-Resident Gao Promotes Protrusive Membrane Dynamics Cell. 2017 PMID: 28803726 |
3. The Gao Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons Neural Plast. 2016 PMID: 26881110 |
4. Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation J Biol Chem. 2016 PMID: 27402827 |
5. Toll-like receptor 2-mediated MAPKs and NF-?B activation requires the GNAO1-dependent pathway in human mast cells Integr Biol (Camb). 2016 PMID: 27515449 |
6. Go is required for the release of IL-8 and TNF-a, but not degranulation in human mast cells Eur J Pharmacol. 2016 PMID: 27025291 |
7. AKT-independent Reelin signaling requires interactions of heterotrimeric Go and Src Biochem Biophys Res Commun. 2015 PMID: 26441085 |