| 货号: N263175 |
| 产品全名: Phospho-MCM2 (Ser27) 兔单克隆抗体 |
| 基因符号 MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 |
| UNIPROT ID: P49736 |
| 背景: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] |
| 抗原: A synthetic phosphopeptide corresponding to residues surrounding Ser27 of human MCM2 |
| 经过测试的应用: WB,IHC-F,IHC-P,ICC/IF,IP |
| 推荐稀释比: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Monoclonal |
| 克隆编号: R08-4D8 |
| 分子量: Calculated MW: 102 kDa; Observed MW: 125 kDa |
| 亚型: IgG |
| 纯化: Affinity Purified |
| 种属反应性: Human |
| Modification: Phosphorylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin-embedded Human tonsil using MCM2 (Phospho-Ser27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. | 
Immunocytochemistry analysis of Phospho-MCM2 (Ser27) (green) in Hela using Phospho-MCM2 (Ser27) antibody,and DAPI(blue). | 
Western blot analysis of MCM2 (Phospho-Ser27) in Hela, A549, HL-60, U2OS lysates using MCM2 (Phospho-Ser27) antibody. | |
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