| 货号: N225421 |
| 产品全名: Phospho-FGFR1 (Tyr654) 兔多抗 |
| 基因符号 FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD antigen CD331 |
| UNIPROT ID: P11362 |
| 背景: Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain. Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues. Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. |
| 抗原: The antiserum was produced against synthesized peptide derived from human FGFR1 around the phosphorylation site of Tyr654. AA range:626-675 |
| 经过测试的应用: WB,ICC/IF,ELISA |
| 推荐稀释比: WB: 1/500-1/1000 IF: 1/50-1/200 ELISA: 1/10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 分子量: Calculated MW: 92 kDa; Observed MW: 120 kDa |
| 亚型: IgG |
| 纯化: Affinity Chromatography |
| 种属反应性: Human, Mouse and Rat |
| Modification: Phosphorylated |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
| 研究领域: Cardiovascular |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
EnzymeLinked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho-peptide (Phospho-left) and NonPhospho-peptide (Phospho-right), using FGFR1 (Phospho-Tyr654) antibody. | 
Western blot analysis of Phospho-FGFR1 (Tyr654) in Hela lysates using Phospho-FGFR1 (Tyr654) antibody. | 
Immunofluorescence analysis of Phospho-FGFR1 (Tyr654) in COS7 cells using FGFR1 (Phospho-Tyr654) antibody. The picture on the right is blocked with the Phospho- peptide. | 
Western blot analysis of Phospho-FGFR1 (Tyr654) in 293 lysates treated with Insulin using Phospho-FGFR1 (Tyr654) antibody.The lane on the right is blocked with the Phospho- peptide. |
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