武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

邮箱:2716620741@qq.com

咨询:027 – 63497286 

Phospho-ErbB 2 (Tyr1221/Tyr1222) Rabbit pAb

Anti-Phospho-ErbB 2 (Tyr1221/Tyr1222) Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:N225418

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   N225418
产品全名:   Phospho-ErbB 2 (Tyr1221/Tyr1222) 兔多抗
基因符号   ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340
UNIPROT ID:   P04626
背景:   This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.
抗原:   The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222. AA range:1191-1240
经过测试的应用:   WB,IHC-P,IP,ELISA
推荐稀释比:   WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 ELISA: 1/10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
分子量:   Calculated MW: 138 kDa; Observed MW: 180 kDa
亚型:   IgG
纯化:   Affinity Chromatography
种属反应性:   Human, Mouse and Rat
Modification:   Phosphorylated
成分:   PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
研究领域:   Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr122, 2) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.

Western blot analysis of Phospho-ErbB 2 (Tyr1221/Tyr1222) in various lysates using Phospho-ErbB 2 (Tyr1221/Tyr1222) antibody.

Western blot analysis of Phospho-ErbB 2 (Tyr1221/Tyr1222) in SKOV-3 lysates treated with EGF, using HER2 (Phospho-Tyr1221/Tyr122, 2) antibody. The lane on the right is blocked with the Phospho- peptide.
           引用文献