武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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YAP1 Rabbit pAb

Anti-YAP1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S222449

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S222449
产品全名:   YAP1 兔多抗
基因符号   YAP; YKI; COB1; YAP2; YAP-1; YAP65
UNIPROT ID:   P46937 (Gene Accession – NP_001123617 )
背景:   This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
抗原:   Synthetic peptide of human YAP1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-100;WB: 500-2000;ELISA: 1000-2000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Signal Transduction, Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 222449(YAP1 Antibody) at a dilution of 1/50(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 222449(Anti-YAP1 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: MCF7 cell lysate;
Primary antibody: 222449(YAP1 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 45 seconds
           引用文献