| 货号: S222263 |
| 产品全名: JAM3 兔多抗 |
| 基因符号 JAMC; JAM-2; JAM-3; JAM-C |
| UNIPROT ID: Q9BX67 (Gene Accession – NP_116190 ) |
| 背景: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants. |
| 抗原: Synthetic peptide of human JAM3 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 40-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cardiovascular, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 222263(JAM3 Antibody) at a dilution of 1/45(Cytoplasm ). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 222263(Anti-JAM3 Antibody) at dilution 1/45. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: K562 cell lysate;
Primary antibody: 222263(JAM3 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 7 minutes | |
|
