| 货号: S222052 |
| 产品全名: FUBP1 兔多抗 |
| 基因符号 FBP; FUBP; hDH V |
| UNIPROT ID: Q96AE4 (Gene Accession – NP_003893 ) |
| 背景: The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3′-5′ helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. |
| 抗原: Synthetic peptide of human FUBP1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-50;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 222052(FUBP1 Antibody) at a dilution of 1/25(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 222052(Anti-FUBP1 Antibody) at dilution 1/25. | 
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 222052(Anti-FUBP1 Antibody) at a dilution of 1/25. | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D263947(Anti-FUBP1 Antibody) at dilution 1/25. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cell lysate;
Primary antibody: 222052(FUBP1 Antibody) at dilution 1/650;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 minutes | |
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