| 货号: S221891 |
| 产品全名: H2BC1 兔多抗 |
| 基因符号 STBP; TH2B; H2BFU; TSH2B; hTSH2B; TSH2B.1; HIST1H2BA; bA317E16.3 |
| UNIPROT ID: Q96A08 (Gene Accession – NP_733759 ) |
| 背景: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015] |
| 抗原: Synthetic peptide of human H2BC1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221891(H2BC1 Antibody) at a dilution of 1/45(Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221891(Anti-H2BC1 Antibody) at dilution 1/45. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 221891(Anti-H2BC1 Antibody) at a dilution of 1/45. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D263694(Anti-H2BC1 Antibody) at dilution 1/45. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Human prostate tissue, PC-3, A549 and TM4 cell lysates;
Primary antibody: 221891(H2BC1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds | |
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