| 货号: S221811 |
| 产品全名: PRPF8 兔多抗 |
| 基因符号 PRP8; RP13; HPRP8; PRPC8; SNRNP220 |
| UNIPROT ID: Q6P2Q9 (Gene Accession – NP_006436 ) |
| 背景: Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. |
| 抗原: Synthetic peptide of human PRPF8 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 20-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 221811(PRPF8 Antibody) at a dilution of 1/20(Nucleus or Cytoplasm ). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 221811(Anti-PRPF8 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 221811(Anti-PRPF8 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D263565(Anti-PRPF8 Antibody) at dilution 1/20. | 
Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: 293T, K562, Raji, Hela and NIH/3T3 cell lysates;
Primary antibody: 221811(PRPF8 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 50 seconds | |
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