| 货号: S221802 |
| 产品全名: RPL14 兔多抗 |
| 基因符号 L14; RL14; hRL14; CTG-B33; CAG-ISL-7 |
| UNIPROT ID: P50914 (Gene Accession – NP_003964 ) |
| 背景: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5′-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| 抗原: Synthetic peptide of human RPL14 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Epigenetics and Nuclear Signaling |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221802(RPL14 Antibody) at a dilution of 1/30(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221802(Anti-RPL14 Antibody) at dilution 1/30. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: A431, HEPG2, 231, Jurkat, Hela and 293T cell lysates;
Primary antibody: 221802(RPL14 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds | |
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