| 货号: S221640 |
| 产品全名: ATP5MPL 兔多抗 |
| 基因符号 MLQ; MP68; PLPM; 6.8PL; C14orf2 |
| UNIPROT ID: P56378 (Gene Accession – NP_004885 ) |
| 背景: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 – containing the extramembraneous catalytic core and F0 – containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). Minor subunit required to maintain the ATP synthase population in the mitochondria (PubMed:24330338). |
| 抗原: Synthetic peptide of human ATP5MPL |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 40-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 221640(ATP5MPL Antibody) at a dilution of 1/60(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 221640(Anti-ATP5MPL Antibody) at dilution 1/60. | 
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 221640(Anti-ATP5MPL Antibody) at a dilution of 1/60. | 
In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D263326(Anti-ATP5MPL Antibody) at dilution 1/60. | 
Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 293T and RAW264.7 cell?Human fetal brain tissue?Jurkat and 231 cell?Human heart tissue?Hela cell lysates;
Primary antibody: 221640(ATP5MPL Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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