| 货号: S221377 |
| 产品全名: IFITM1 兔多抗 |
| 基因符号 9-27; CD225; IFI17; LEU13; DSPA2a |
| UNIPROT ID: P13164 (Gene Accession – NP_003632 ) |
| 背景: The IFITM family of proteins are transmembrane proteins so named because their expression is IFN-inducible. IFITM proteins have been found upregulated in human colorectal carcinomas. Both mouse IFITM1 (also known as CD225) and IFITM3 demonstrate expression on the cell surfaces of primordial germ cells in a developmentally-regulated manner. They presumably modulate cell adhesion and influence cell differentiation. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm. |
| 抗原: Synthetic peptide of human IFITM1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-100;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Cancer, Immunology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221377(IFITM1 Antibody) at a dilution of 1/20(Cytoplasm and Cell membrane). | 
In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221377(Anti-IFITM1 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 221377(Anti-IFITM1 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D262939(Anti-IFITM1 Antibody) at dilution 1/20. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and NIH/3T3 cell lysates;
Primary antibody: 221377(IFITM1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds | |
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