武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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GLS Rabbit pAb

Anti-GLS Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S220577

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S220577
产品全名:   GLS 兔多抗
基因符号   GAC; GAM; KGA; GLS1; AAD20
UNIPROT ID:   O94925 (Gene Accession – NP_001243239 )
背景:   This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
抗原:   Synthetic peptide of human GLS
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism, Neuroscience
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220577(GLS Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220577(Anti-GLS Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and HT-29 cell lysates;
Primary antibody: 220577(GLS Antibody) at dilution 1/650;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
           引用文献