| 货号: S220488 |
| 产品全名: CYP46A1 兔多抗 |
| 基因符号 CP46; CYP46 |
| UNIPROT ID: Q9Y6A2 (Gene Accession – NP_006659 ) |
| 背景: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008] |
| 抗原: Synthetic peptide of human CYP46A1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-100;WB: 1000-5000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Metabolism, Cancer, Neuroscience |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 220488(CYP46A1 Antibody) at a dilution of 1/50(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 220488(Anti-CYP46A1 Antibody) at dilution 1/50. | 
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220488(Anti-CYP46A1 Antibody) at a dilution of 1/50. | 
In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261599(Anti-CYP46A1 Antibody) at dilution 1/50. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 231, RAMOS, MCF-7 cell lysates;
Primary antibody: 220488(CYP46A1 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds | |
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