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CDC27 Rabbit pAb

Anti-CDC27 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S220447

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S220447
产品全名:   CDC27 兔多抗
基因符号   APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E
UNIPROT ID:   P30260 (Gene Accession – NP_001107563 )
背景:   The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.
抗原:   Synthetic peptide of human CDC27
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cancer, Cell Biology
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 220447(CDC27 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 220447(Anti-CDC27 Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 cells, K562 cells;
Primary antibody: 220447(CDC27 Antibody) at dilution 1/750;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
           引用文献