| 货号: S219979 |
| 产品全名: RASSF1 兔多抗 |
| 基因符号 123F2; RDA32; NORE2A; RASSF1A; REH3P21 |
| UNIPROT ID: Q9NS23 (Gene Accession – NP_733830 ) |
| 背景: This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| 抗原: Synthetic peptide of human RASSF1 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 25-50;WB: 200-1000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction, Epigenetics and Nuclear Signaling, Cancer |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219979(RASSF1 Antibody) at a dilution of 1/20(Cytoplasm). | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 219979(Anti-RASSF1 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 219979(Anti-RASSF1 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D260726(Anti-RASSF1 Antibody) at dilution 1/20. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 219979(RASSF1 Antibody) at dilution 1/250;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes | |
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