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GSDMA Rabbit pAb

Anti-GSDMA Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S219464

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S219464
产品全名:   GSDMA 兔多抗
基因符号   GSDM; FKSG9; GSDM1
UNIPROT ID:   Q96QA5 (Gene Accession – BC109197 )
背景:   May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown (Probable).
抗原:   Fusion protein of human GSDMA
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 150-300;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Cancer
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 219464(GSDMA Antibody) at a dilution of 1/150(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 219464(Anti-GSDMA Antibody) at dilution 1/150.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, Hela and LO2 cell lysates;
Primary antibody: 219464(GSDMA Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
           引用文献