| 货号: S219130 |
| 产品全名: RRP8 兔多抗 |
| 基因符号 NML; KIAA0409 |
| UNIPROT ID: O43159 (Gene Accession – BC001071 ) |
| 背景: Essential component of the eNoSC (energy-dependent nucleolar silencing) comple,x a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at ‘Lys-9’ (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the comple,x RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. |
| 抗原: Fusion protein of human RRP8 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 50-300;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219130(RRP8 Antibody) at a dilution of 1/80(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 219130(Anti-RRP8 Antibody) at dilution 1/80. | 
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 219130(Anti-RRP8 Antibody) at a dilution of 1/80. | 
In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D225896(Anti-RRP8 Antibody) at dilution 1/80. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human cerebella tissue, K562 cell lysates;
Primary antibody: 219130(RRP8 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds | |
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