| 货号: S218885 |
| 产品全名: PSMD12 兔多抗 |
| 基因符号 p55; Rpn5; STISS |
| UNIPROT ID: O00232 (Gene Accession – BC019062 ) |
| 背景: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 抗原: Fusion protein of human PSMD12 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 40-200;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Cell Biology |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 218885(PSMD12 Antibody) at a dilution of 1/40(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 218885(Anti-PSMD12 Antibody) at dilution 1/40. | 
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 218885(Anti-PSMD12 Antibody) at a dilution of 1/40. | 
In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D225437(Anti-PSMD12 Antibody) at dilution 1/40. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Rat heart tissue lysate;
Primary antibody: 218885(PSMD12 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute | |
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