| 货号: S218762 |
| 产品全名: HSPA2 兔多抗 |
| 基因符号 HSP70-2; HSP70-3 |
| UNIPROT ID: P54652 (Gene Accession – BC036107 ) |
| 背景: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells. |
| 抗原: Fusion protein of human HSPA2 |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: IHC: 30-150;WB: 500-2000;ELISA: 5000-10000 |
| 种属反应性: Rabbit |
| 克隆性： Rabbit Polyclonal |
| 亚型: Immunogen-specific rabbit IgG |
| 纯化: Antigen affinity purification |
| 种属反应性: Human, Mouse, Rat |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
| 研究领域: Signal Transduction |
| 储存和运输: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 218762(HSPA2 Antibody) at a dilution of 1/20(Cytoplasm and Nucleus). | 
In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 218762(Anti-HSPA2 Antibody) at dilution 1/20. | 
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 218762(Anti-HSPA2 Antibody) at a dilution of 1/20. | 
In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with fusion protein and then with D225129(Anti-HSPA2 Antibody) at dilution 1/20. | 
Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 231 cell, Hela cell, Jurkat cell, A549 cell, NIH/3T3 cell, Human cerebrum tissue, Mouse brain tissue lysates;
Primary antibody: 218762(HSPA2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds | |
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