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SHMT2 Rabbit pAb

Anti-SHMT2 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S218745

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S218745
产品全名:   SHMT2 兔多抗
基因符号   GLYA; SHMT; HEL-S-51e
UNIPROT ID:   P34897 (Gene Accession – BC011911 )
背景:   This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
抗原:   Fusion protein of human SHMT2
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 30-150;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Metabolism
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 218745(SHMT2 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 218745(Anti-SHMT2 Antibody) at dilution 1/20.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Jurkat, Raji, 293T and HEPG2 cell lysates;
Primary antibody: 218745(SHMT2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
           引用文献