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DDX59 Rabbit pAb

Anti-DDX59 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S218597

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S218597
产品全名:   DDX59 兔多抗
基因符号   OFD5; ZNHIT5
UNIPROT ID:   Q5T1V6 (Gene Accession – BC041801 )
背景:   DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX59 (DEAD box protein 59), also known as ZNHIT5 (zinc finger HIT domaincontaining protein 5), is a 619 amino acid member of the DEAD box helicase protein family. Like many DEAD box helicase family members, DDX59 contains a Q motif, which controls ATP binding and hydrolysis. Expressed as two isoforms produced by alternative splicing, DDX59 contains one helicase C-terminal domain and one HIT-type zinc finger.
抗原:   Fusion protein of human DDX59
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse, Rat
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218597(DDX59 Antibody) at a dilution of 1/30(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218597(Anti-DDX59 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A549 and 293T cell lysates;
Primary antibody: 218597(DDX59 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 minutes
           引用文献