武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

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U2AF1 Rabbit pAb

Anti-U2AF1 Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S218300

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S218300
产品全名:   U2AF1 兔多抗
基因符号   RN; FP793; U2AF35; U2AFBP; RNU2AF1
UNIPROT ID:   Q01081 (Gene Accession – BC001177 )
背景:   This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
抗原:   Fusion protein of human U2AF1
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218300(U2AF1 Antibody) at a dilution of 1/130(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218300(Anti-U2AF1 Antibody) at dilution 1/130.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 231 and HepG2 cell lysates;
Primary antibody: 218300(U2AF1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
           引用文献